Journal:

Article Title: Differentiation of Lactobacillus Species by Molecular Typing

doi:

Figure Lengend Snippet: Molecular typing of Lactobacillus isolates with respect to ATCC typed strains

Article Snippet: The nomenclature used here for the type and reference strains was that given by ATCC and NCFB prior to 1993, and that used for the clinical and stock isolates was the same as that reported in publications by our group ( 23 , 24 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Original nomenclature Ribotype with Bcl I Chromotype with Bcl I L. rhamnosus ATCC 7469 T A 1.1 L. rhamnosus ATCC 11443 A 1.1 L. casei subsp. rhamnosus 81 A 1.1 L. casei subsp. rhamnosus 76 A 1.2 L. rhamnosus ATCC 8530 A 1.3 L. casei subsp. rhamnosus GR-1 A 1.4 L. casei subsp. rhamnosus 36 A 1.4 Vaginal isolate w A 1.4 Vaginal isolate g A 1.4 L. rhamnosus NCFB 2964 A 1.5 L. casei subsp. rhamnosus RC-17 A 1.6 L. fermentum A60 A 1.6 L. plantarum RC-6 A 1.7 L. rhamnosus ATCC 21052 A 2.1 C1-A A 2.1 C3-A A 2.1 C3-B A 2.1 C5-A A 2.1 C7-A A 2.1 C7-B A 2.1 L. casei subsp. casei ATCC 393 B 10.1 L. casei subsp. casei ATCC 4940 B1 11.1 L. paracasei subsp. paracasei ATCC 25302 T C 20.1 L. casei subsp. casei ATCC 334 C 20.2 L. casei subsp. casei ATCC 4007 C1 21.1 L. casei subsp. casei ATCC 4913 C2 22.1 L. casei subsp. casei 55 C2 23.1 L. paracasei subsp. paracasei ATCC 25303 C2 24.1 L. casei subsp. rhamnosus 43 C3 25.1 L. casei 8 C3 25.1 L. casei 65 C3 25.1 L. plantarum ATCC 14917 T D 30.1 L. plantarum UH 2153 D 30.1 L. plantarum ATCC 10012 D 30.2 L. plantarum ATCC 11974 D1 31.1 L. plantarum ATCC 14431 D2 32.1 L. plantarum ATCC 8014 D3 33.1 L. plantarum 260 D3 33.1 L. plantarum RC-20 D4 34.1 L. acidophilus 75 D5 34.2 US2-A D6 35.1 US2-B D6 35.1 US2-C D6 35.1 US3-A D7 35.2 US3-B D7 35.2 US6-A D8 36.1 US6-B D8 36.1 US6-C D8 36.1 C4-A D8 36.1 C4-B D8 36.1 US8-A D9 37.1 L. fermentum ATCC 14931 T E 40.1 L. fermentum ATCC 23271 E1 40.2 L. fermentum ATCC 8289 E2 40.3 L. fermentum ATCC 9338 E2 40.3 L. fermentum ATCC 11739 E3 41.1 L. fermentum ATCC 14932 E4 42.1 L. fermentum B-54 E5 43.1 L. acidophilus RC-14 E5 43.1 L. acidophilus RC-14 (subcultured 20 times) E5 43.1 L. acidophilus ATCC 4356 T F 50.1 US4 F 50.2 L. acidophilus T-13 F1 51.1 L. jensenii ATCC 25258 T G 60.1 Open in a separate window Molecular typing of Lactobacillus isolates with respect to ATCC typed strains Nine health food products were examined.

Techniques:

Journal:

Article Title: Differentiation of Lactobacillus Species by Molecular Typing

doi:

Figure Lengend Snippet: Autoradiogram of Southern blot of BclI-digested DNA after hybridization with 32P-labelled rRNA from E. coli. Lanes 1 to 12 represent ribotype groups A to F. Lane 1, L. rhamnosus ATCC 7469T; lane 2, L. casei subsp. casei ATCC 393; lane 3, L. paracasei subsp. paracasei ATCC 25302T; lane 4, L. casei subsp. casei ATCC 4007; lane 5, L. paracasei subsp. paracasei ATCC 25303; lane 6, L. casei 8; lane 7, L. plantarum ATCC 14917T; lane 8, L. plantarum ATCC 11974; lane 9, L. plantarum ATCC 14431; lane 10, L. fermentum B-54; lane 11, L. acidophilus ATCC 4356T; lane 12, L. acidophilus T-13.

Article Snippet: The nomenclature used here for the type and reference strains was that given by ATCC and NCFB prior to 1993, and that used for the clinical and stock isolates was the same as that reported in publications by our group ( 23 , 24 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Original nomenclature Ribotype with Bcl I Chromotype with Bcl I L. rhamnosus ATCC 7469 T A 1.1 L. rhamnosus ATCC 11443 A 1.1 L. casei subsp. rhamnosus 81 A 1.1 L. casei subsp. rhamnosus 76 A 1.2 L. rhamnosus ATCC 8530 A 1.3 L. casei subsp. rhamnosus GR-1 A 1.4 L. casei subsp. rhamnosus 36 A 1.4 Vaginal isolate w A 1.4 Vaginal isolate g A 1.4 L. rhamnosus NCFB 2964 A 1.5 L. casei subsp. rhamnosus RC-17 A 1.6 L. fermentum A60 A 1.6 L. plantarum RC-6 A 1.7 L. rhamnosus ATCC 21052 A 2.1 C1-A A 2.1 C3-A A 2.1 C3-B A 2.1 C5-A A 2.1 C7-A A 2.1 C7-B A 2.1 L. casei subsp. casei ATCC 393 B 10.1 L. casei subsp. casei ATCC 4940 B1 11.1 L. paracasei subsp. paracasei ATCC 25302 T C 20.1 L. casei subsp. casei ATCC 334 C 20.2 L. casei subsp. casei ATCC 4007 C1 21.1 L. casei subsp. casei ATCC 4913 C2 22.1 L. casei subsp. casei 55 C2 23.1 L. paracasei subsp. paracasei ATCC 25303 C2 24.1 L. casei subsp. rhamnosus 43 C3 25.1 L. casei 8 C3 25.1 L. casei 65 C3 25.1 L. plantarum ATCC 14917 T D 30.1 L. plantarum UH 2153 D 30.1 L. plantarum ATCC 10012 D 30.2 L. plantarum ATCC 11974 D1 31.1 L. plantarum ATCC 14431 D2 32.1 L. plantarum ATCC 8014 D3 33.1 L. plantarum 260 D3 33.1 L. plantarum RC-20 D4 34.1 L. acidophilus 75 D5 34.2 US2-A D6 35.1 US2-B D6 35.1 US2-C D6 35.1 US3-A D7 35.2 US3-B D7 35.2 US6-A D8 36.1 US6-B D8 36.1 US6-C D8 36.1 C4-A D8 36.1 C4-B D8 36.1 US8-A D9 37.1 L. fermentum ATCC 14931 T E 40.1 L. fermentum ATCC 23271 E1 40.2 L. fermentum ATCC 8289 E2 40.3 L. fermentum ATCC 9338 E2 40.3 L. fermentum ATCC 11739 E3 41.1 L. fermentum ATCC 14932 E4 42.1 L. fermentum B-54 E5 43.1 L. acidophilus RC-14 E5 43.1 L. acidophilus RC-14 (subcultured 20 times) E5 43.1 L. acidophilus ATCC 4356 T F 50.1 US4 F 50.2 L. acidophilus T-13 F1 51.1 L. jensenii ATCC 25258 T G 60.1 Open in a separate window Molecular typing of Lactobacillus isolates with respect to ATCC typed strains Nine health food products were examined.

Techniques: Southern Blot, Hybridization

Journal: BMC Cancer

Article Title: Lactobacillus casei combined with Lactobacillus reuteri alleviate pancreatic cancer by inhibiting TLR4 to promote macrophage M1 polarization and regulate gut microbial homeostasis

doi: 10.1186/s12885-023-11557-z

Figure Lengend Snippet: L. casei & L. reuteri inhibits the proliferation, migration, invasion of pancreatic cancer cells and pancreatic cancer cell-induced M2 polarization of macrophages. ( A ) MTT assay of MIA PaCa-2, Panc-1, BxPC-3, and AsPC-1 cells. ( B ) RT-qPCR analysis of TLR4 and MyD88 expressions in BxPC-3 cells. ( C ) CCK-8 assay of BxPC-3 cells. ( D ) Transwell migration and invasion assay of BxPC-3 cells. ( E ) Representative chart of flow cytometric analysis of CD86 + or CD206 + macrophages. ( F ) Statistical chart of flow cytometric analysis of CD86 + or CD206 + macrophages. ( G ) WB analysis of Arg-1 and iNOS in macrophages. Lactobacillus represents cells treated with L. casei & L. reuteri. * p < 0.05

Article Snippet: L. casei (ATCC 39392) and L. reuteri (ATCC 23272) were purchased from the American Type Culture Collection.

Techniques: Migration, MTT Assay, Quantitative RT-PCR, CCK-8 Assay, Invasion Assay

Journal: BMC Cancer

Article Title: Lactobacillus casei combined with Lactobacillus reuteri alleviate pancreatic cancer by inhibiting TLR4 to promote macrophage M1 polarization and regulate gut microbial homeostasis

doi: 10.1186/s12885-023-11557-z

Figure Lengend Snippet: L. casei & L. reuteri influences pancreatic cancer cells and macrophage polarization by regulating TLR4. ( A ) RT-qPCR analysis of TLR4 and MyD88 expressions in BxPC-3 cells. ( B ) Statistical chart of flow cytometric analysis of CD86 + or CD206 + macrophages. ( C ) Representative chart of flow cytometric analysis of CD86 + or CD206 + macrophages. ( D ) WB analysis of Arg-1 and iNOS in macrophages. ( E ) CCK-8 assay of BxPC-3 cells. ( F ) Transwell migration and invasion assay of BxPC-3 cells. * p < 0.05 versus control group, # p < 0.05 versus LPS group

Article Snippet: L. casei (ATCC 39392) and L. reuteri (ATCC 23272) were purchased from the American Type Culture Collection.

Techniques: Quantitative RT-PCR, CCK-8 Assay, Migration, Invasion Assay, Control

Journal: BMC Cancer

Article Title: Lactobacillus casei combined with Lactobacillus reuteri alleviate pancreatic cancer by inhibiting TLR4 to promote macrophage M1 polarization and regulate gut microbial homeostasis

doi: 10.1186/s12885-023-11557-z

Figure Lengend Snippet: L. casei & L. reuteri represses pancreatic cancer growth and promotes M1 macrophage polarization by inhibiting TLR4. ( A ) Nude mice were xenografted with BxPC-3 cells (PC group) and treated with L. casei & L. reuteri (Lactobacillus group) or TAK-242 (TAK-242 group). The tumor sizes are shown. ( B ) The tumor volumes (left) and tumor weights (right) of nude mice. ( C ) Body weights of nude mice. ( D ) RT-qPCR analysis of TLR4 and MyD88 expressions in tumor tissues. ( E ) IHC staining for Ki67 in tumor tissues. Scale bar: 100 μm (upper), 25 μm (lower). ( F ) IF staining for CD86 and CD206 in tumor tissues. ( G ) IF staining for Arg-1 and iNOS in tumor tissues. ( H ) Flow cytometric analysis of CD86 + or CD206 + macrophages in tumor tissues. * p < 0.05 versus PC group

Article Snippet: L. casei (ATCC 39392) and L. reuteri (ATCC 23272) were purchased from the American Type Culture Collection.

Techniques: Quantitative RT-PCR, Immunohistochemistry, Staining

Journal: BMC Cancer

Article Title: Lactobacillus casei combined with Lactobacillus reuteri alleviate pancreatic cancer by inhibiting TLR4 to promote macrophage M1 polarization and regulate gut microbial homeostasis

doi: 10.1186/s12885-023-11557-z

Figure Lengend Snippet: L. casei & L. reuteri regulates gut microbial homeostasis by inhibiting TLR4. ( A ) ASV analysis of the relative abundance of species. ( B ) Alpha diversity of gut microbiota. ( C ) PCoA analysis of gut microbiota. ( D ) Beta diversity of gut microbiota. ( E ) The relative abundance of gut microbiota at phylum (left) and genus (right) level. ( F ) Differences of relative abundance of specific gut microbiota at genus level. * p < 0.05 versus control group, # p < 0.05 versus PC group

Article Snippet: L. casei (ATCC 39392) and L. reuteri (ATCC 23272) were purchased from the American Type Culture Collection.

Techniques: Control

Journal: BMC Cancer

Article Title: Lactobacillus casei combined with Lactobacillus reuteri alleviate pancreatic cancer by inhibiting TLR4 to promote macrophage M1 polarization and regulate gut microbial homeostasis

doi: 10.1186/s12885-023-11557-z

Figure Lengend Snippet: L. casei & L. reuteri regulates gut microbial homeostasis by inhibiting TLR4. ( A ) PCA scores plot for metabolic profiles. ( B ) PLS-DA plot for metabolic profiles. ( C ) The heat map of the metabolic features

Article Snippet: L. casei (ATCC 39392) and L. reuteri (ATCC 23272) were purchased from the American Type Culture Collection.

Techniques: